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Nuclear localization of orphan receptor protein kinase (Ror1) is mediated through the juxtamembrane domain by Hsiao-Chun Tseng; Ping-Chiang Lyu; Wen-chang Lin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Background Several receptor tyrosine kinases (RTKs) such as EGFR, FGFR, TRK, and VEGFR are capable of localizing in the cell nucleus in addition to their usual plasma membrane localization. Recent reports also demonstrate that nuclear-localized RTKs have important cellular functions such as transcriptional activation. On the basis of preliminary bioinformatic analysis, additional RTKs, including receptor tyrosine kinase-like orphan receptor 1 (Ror1) were predicted to have the potential for nuclear subcellular localization. Ror1 is a receptor protein tyrosine kinase that modulates neurite growth in the central nervous system. Because the nuclear localization capability of the Ror1 cytoplasmic domain has not been reported, we examined the cellular expression distribution of this region. ## Results The Ror1 cytoplasmic region was amplified and cloned into reporter constructs with fluorescent tags. Following transfection, the nuclear distribution patterns of transiently expressed fusion proteins were observed. Serial deletion constructs were then used to map the juxtamembrane domain of Ror1 (aa\_471-513) for this nuclear translocation activity. Further site-directed muta

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Author

Hsiao-Chun Tseng; Ping-Chiang Lyu; Wen-chang Lin

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-2018.; Springer Science and Business Media LLC (ISSN 1471-2121)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)