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Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling by Yuma Ito; Takashi Hirasawa; Hiroshi Shimizu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract We performed metabolic engineering on the budding yeast Saccharomyces cerevisiae for enhanced production of succinic acid. Aerobic succinic acid production in S. cerevisiae was achieved by disrupting the SDH1 and SDH2 genes, which encode the catalytic subunits of succinic acid dehydrogenase. Increased succinic acid production was achieved by eliminating the ethanol biosynthesis pathways. Metabolic profiling analysis revealed that succinic acid accumulated intracellularly following disruption of the SDH1 and SDH2 genes, which suggests that enhancing the export of intracellular succinic acid outside of cells increases succinic acid production in S. cerevisiae. The mae1 gene encoding the Schizosaccharomyces pombe malic acid transporter was introduced into S. cerevisiae, and as a result, succinic acid production was successfully improved. Metabolic profiling analysis is useful in producing chemicals for metabolic engineering of microorganisms.

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Author

Yuma Ito; Takashi Hirasawa; Hiroshi Shimizu

Publisher

Informa UK Limited

Published

2014

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)