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Human Herpesvirus Type 2 Infection of Primary Murine Astrocytes Causes Disruption of the Mitochondrial Network and Remodeling of the Actin Cytoskeleton: an in Vitro Morphological Study by Anna Słońska; Joanna Cymerys; Marcin Chodkowski; Piotr Bąska; Małgorzata Krzyżowska; Marcin W. Bańbura is a Medicine article available to read on EtoBox.
What is Human Herpesvirus Type 2 Infection of Primary Murine Astrocytes Causes Disruption of the Mitochondrial Network and Remodeling of the Actin Cytoskeleton: an in Vitro Morphological Study about?
Herpesviruses are capable of infecting not only neurons, where they establish latent infection, but also astrocytes. Since astrocytes are important for the functioning of the central nervous system (CNS), their infection may lead to serious neurological disorders. Thus, in the present study we investigated the ability of human herpesvirus type 2 (HHV-2) to infect primary murine astrocytes __in vitro__ and the effect of infection on their mitochondrial network and actin cytoskeleton. In immunofluorescence assays, antibodies against HHV-2 antigens and glial fibrillary acidic protein (GFAP) were used to confirm that the infected cells are indeed astrocytes. Real-time PCR analysis showed a high level of HHV-2 replication in astrocytes, particularly at 168 h postinfection, confirming that a productive infection had occurred. Analysis of mitochondrial morphology showed that, starting from the first stage of infection, HHV-2 caused fragmentation of the mitochondrial network and formation of punctate and tubular structures that colocalized with virus particles. Furthermore, during the late stages of infection, the infection affected the actin cytoskeleton and induced formation of actin-bas
Who reads Human Herpesvirus Type 2 Infection of Primary Murine Astrocytes Causes Disruption of the Mitochondrial Network and Remodeling of the Actin Cytoskeleton: an in Vitro Morphological Study?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Anna Słońska; Joanna Cymerys; Marcin Chodkowski; Piotr Bąska; Małgorzata Krzyżowska; Marcin W. Bańbura
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Medicine (Life Sciences)