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What is Replica Plating for Mutant Isolation about?

This document describes the replica-plating technique developed by Esther and Joshua Lederberg in 1952. The technique allows for the transfer of bacterial colonies from a primary "master" plate onto multiple secondary "replica" plates through the use of a velvet cloth. This allows for the detection and isolation of bacterial mutants through their growth on selective media. Specifically, auxotrophic mutants that fail to grow on minimal media can be identified and isolated for further study through their abse

Author

PUSHPALATHA.T

Language

EN