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New sites of methylcytosine-rich DNA detected on metaphase chromosomes by Agnès Barbin; Claire Montpellier; Nadja Kokalj-Vokac; Anne Gibaud; Alain Niveleau; Bernard Malfoy; Bernard Dutrillaux; Claire A. Bourgeois is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is New sites of methylcytosine-rich DNA detected on metaphase chromosomes about?

In situ immunofluorescence detection of antibodies against 5-methylcytosine on metaphase chromosomes prepared by a new procedure allows the display of new 5-methylcytosine-rich sites as compared to previously published methods. In short-term culture lymphocytes, the immunofluorescent signals give a recurrent pattern in which four types of binding sites can be distinguished. Type I sites are the secondary constrictions and a few juxtacentromeric regions, type II sites correspond to T-bands. Both types I and II sites emit a strong fluorescence. Type III sites form an R-band pattern and emit a weaker fluorescence. Type IV sites are the short arms of acrocentrics, they emit strong but polymorphic signals. The results obtained from control experiments suggest that the pattern observed is rather the expression of an uneven distribution of 5-methylcytosine-rich sites than a consequence of the various treatments used. In a lymphoblastoid cell line known to have a reduced 5-methylcytosine content, it was possible to demonstrate a heterogeneous hypomethylation among chromosome structures, principally involving type I sites. The method opens the possibility of studying in situ on chromosomes,

Who reads New sites of methylcytosine-rich DNA detected on metaphase chromosomes?

Author

Agnès Barbin; Claire Montpellier; Nadja Kokalj-Vokac; Anne Gibaud; Alain Niveleau; Bernard Malfoy; Bernard Dutrillaux; Claire A. Bourgeois

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0340-6717)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)