Opening book details…
Can I read Polyethylene glycol (PEG) embedding and subsequent de-embedding as a method for the structural and immunocytochemical examination of biological specimens by electron microscopy on EtoBox?
Polyethylene glycol (PEG) embedding and subsequent de-embedding as a method for the structural and immunocytochemical examination of biological specimens by electron microscopy by Hisatake Kondo is a Materials Science article available to read on EtoBox.
What is Polyethylene glycol (PEG) embedding and subsequent de-embedding as a method for the structural and immunocytochemical examination of biological specimens by electron microscopy about?
A simple and reliable method to make resinless sections for electron microscopy was recently developed by using polyethylene glycol (PEG) as a transient embedding media. In this paper the practical procedure of this PEG method is described in detail. Normal ultrastructure of several types of in-situ cells in resinless sections is demonstrated. The cytoplasmic matrix of all in-situ cells examined is revealed to consist of the microtrabecular lattice. A result from application of this technique to immuno-electron microscopy is also illustrated. This method is shown to have potential in overcoming the problem of intracellular penetration of macromolecular antibodies. Several artifacts caused by failures in specimen preparations are displayed. The real or artifactual nature of the microtrabecula is briefly discussed.
Who reads Polyethylene glycol (PEG) embedding and subsequent de-embedding as a method for the structural and immunocytochemical examination of biological specimens by electron microscopy?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Hisatake Kondo
- Publisher
- Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0741-0581)
- Published
- 1984
- Language
- EN
- Field
- Materials Science (Physical Sciences)