Can I read A High-resolution Analysis of Chromatin Structure Along P53 Sequences on EtoBox?

A High-resolution Analysis of Chromatin Structure Along P53 Sequences by Silvia Tornaletti; Steven Bates; Gerd P. Pfeifer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A High-resolution Analysis of Chromatin Structure Along P53 Sequences about?

A detailed investigation of how nucleosomes are formed and arranged on the DNA sequence is a prerequisite to understanding the molecular mechanisms of DNA-dependent processes such as transcription, replication, DNA repair, and mutagenesis. In this report we analyzed the chromatin structure of exons 5-8 of thep53 gene in human fibroblasts. We mapped at the nucleotide level the positions of DNase I and micrococcal nuclease cleavage sites in permeabilized cells. Areas of clear DNase I protection, which would be indicative of the binding of sequence-specific proteins, were not detected. Instead, the micrococcal nuclease and DNase digestion patterns suggested that this region was covered by nucleosornes and that two areas spanning exons 5 and 6 are occupied preferentially. These nucleosomes could influence DNA damage distribution, repair of certain lesions, and other aspects of the mutagenesis process in p53 sequences.

Who reads A High-resolution Analysis of Chromatin Structure Along P53 Sequences?

Author

Silvia Tornaletti; Steven Bates; Gerd P. Pfeifer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)