Can I read Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice on EtoBox?

Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice by Xuliang Zhang; Lixin Xu; Xiaokai Song; Xiangrui Li; Ruofeng Yan is a Immunology and Microbiology article available to read on EtoBox.

What is Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice about?

## Abstract __Trichinella spiralis__, the main pathogen of trichinosis, infects a wide range of mammalian hosts and is one of the most widespread parasites worldwide. For parasites, glycolysis is the most important way to generate energy. Previous studies showed that some enzymes involved in the glycolytic pathway play roles in regulation the host immunity. In this paper, enolase from __T. spiralis__ was cloned and the protective potentials were studied. One hundred and sixty ICR mice were divided into four groups and vaccinated with recombinant enolase (pET-ENO), eukaryotic recombinant plasmid encoding enolase (pVAX1-ENO) and negative controls (pVAXl and PBS), respectively. Two weeks after the second immunization, each mouse was challenged orally with 200 muscle larvae (MLs) of __T. spiralis__. Results showed that mice vaccinated with pET-ENO and pVAX1-ENO induced specific antibodies of IgG, IgA, IgM, but no IgE. Subclasses of IgG antibodies showed that mice immunized with recombinant protein and recombinant plasmids induced a Th1/Th2 immune response. Concentrations of serum cytokines were detected and showed significant increase of IFN-γ, IL-4 and TGFβ1, while IL-17 in each group

Who reads Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice?

Author

Xuliang Zhang; Lixin Xu; Xiaokai Song; Xiangrui Li; Ruofeng Yan

Publisher

Springer Science and Business Media LLC

Published

2018

Language

EN

Field

Immunology and Microbiology (Life Sciences)