Can I read N-Methyl-d-Aspartic Acid Stimulation of Vasopressin Release: Role in Osmotic Regulation and Modulation by Gonadal Steriods on EtoBox?

N-Methyl-d-Aspartic Acid Stimulation of Vasopressin Release: Role in Osmotic Regulation and Modulation by Gonadal Steriods by Kerry L. Swenson; Shenon E. Badre; Delmore J. Morsette; Celia D. Sladek is a Neuroscience article available to read on EtoBox.

What is N-Methyl-d-Aspartic Acid Stimulation of Vasopressin Release: Role in Osmotic Regulation and Modulation by Gonadal Steriods about?

Previous experiments demonstrated that excitatory amino acids participate in the osmotic regulation of vasopressin secretion, but the specific involvement of __N__‐methyl‐ d‐aspartic acid (NMDA) receptors was not evaluated. This was demonstrated in the present studies. NMDA stimulated vasopressin release from perifused explants of the hypothalamo‐neurohypophyseal system (HNS), and osmotic stimulation of vasopressin release was inhibited by MK‐801 (10 μM) and AP5 (100 μM) NMDA receptor antagonists. The effective concentration of NMDA was dependent upon the Mg^2+^ concentration of the perifusate with stimulation observed at 1 μM NMDA in Mg^2+^‐replete compared with 5 μM in low‐Mg^2+^ medium. Previous experiments also demonstrated that estradiol and dihydrotestosterone (DHT) inhibited osmotically stimulated vasopressin secretion, and a nongenomic mechanism of action was suggested by the ability of steroids conjugated to bovine serum albumin to replicate the effect. Experiments were performed to explore the potential role of NMDA receptors in this mechanism. Estradiol (50 pg/ml) and DHT (3 ng/ml) inhibited NMDA stimulated vasopressin release in perifused HNS explants. These results sug

Who reads N-Methyl-d-Aspartic Acid Stimulation of Vasopressin Release: Role in Osmotic Regulation and Modulation by Gonadal Steriods?

Author

Kerry L. Swenson; Shenon E. Badre; Delmore J. Morsette; Celia D. Sladek

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-8194)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)