About this document
Linkers, Adaptors, and Homopolymer Tailing by cannier is a document available to read on EtoBox.
Plasmid vectors are circular DNA molecules that can accept foreign DNA fragments through restriction enzyme cleavage and DNA ligation. When the vector and insert DNA have compatible sticky ends, they can be joined together by DNA ligase to form a recombinant vector. Linkers and adaptors can be used to attach sticky ends to blunt-ended DNA fragments, but homopolymer tailing is more efficient as it prevents self-ligation of the vector. In homopolymer tailing, gene inserts and vectors are tailed with complemen
- Author
- cannier
- Language
- EN