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Mitochondrial aspartate/glutamate carrier AGC1 regulates cardiac function via Drp1-mediated mitochondrial fission in doxorubicin-induced cardiomyopathy 1 INTRODUCTION 1 MATERIALS AND METHODS 2 Myocardial samples from failing human and normal hearts 2 Immunoprecipitation 2 In-gel digestion 2 Proteomics analysis by liquid chromatography-mass spectrometry (LC-MS) /MS 2 Database searching 2 Mouse models 3 Virus preparation 3 Transthoracic echocardiography 3 Electron microscopy assessment on mitochondrial morphology 3 Cell culture 3 Cell viral transfection 3 Annexin/PI staining assay 3 Detection of reactive oxygen species (ROS) 3 Mitochondrial potential (DeltaPsim) determination 4 Cytoplasmic and mitochondrial protein preparation 4 Intracellular ATP detection 4 Immunohistochemistry analysis 4 Wheat germ agglutinin (WGA) staining 4 Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining 4 Western blot analysis 4 Statistical analyses 4 RESULTS 4 Mitochondrial inner membrane protein AGC1 is upregulated in the human failing heart 4 Knockdown of AGC1 Ameliorates DOX-Induced Cardiomyopathy 6 Knockdown of AGC1 alleviates DOX-Induced cardiac apoptosis 6 AGC1 knock
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Common subject areas: history, science, philosophy, social sciences.
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- nonfiction