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Detoxification of 4‐hydroxynonenal (HNE) in keratinocytes: characterization of conjugated metabolites by liquid chromatography/electrospray ionization tandem mass spectrometry by Giancarlo Aldini; Paola Granata; Marica Orioli; Enzo Santaniello; Marina Carini is a Chemistry article available to read on EtoBox.

What is Detoxification of 4‐hydroxynonenal (HNE) in keratinocytes: characterization of conjugated metabolites by liquid chromatography/electrospray ionization tandem mass spectrometry about?

## Abstract Keratinocytes are potential targets of lipid peroxidation products (α,β‐unsaturated aldehydes) generated in the skin following UV exposure, among which the most abundant and toxic product is 4‐hydroxy‐__trans__‐2,3‐nonenal (HNE). The aim of this study was to investigate the ability of keratinocytes (NCTC2544 cell lines) to detoxify HNE, through characterization of metabolites, until now never demonstrated, using a combined analytical approach (liquid chromatography (LC) and liquid chromatography/mass spectrometry (LC/MS)). Incubation of cells with HNE (up to 200 μM) was performed in order to evaluate the ability of the cells to detoxify this toxic aldehyde, and indicated that the cell viability was maintained under these conditions. LC analysis of the extracellular media from keratinocytes incubated with 100 μM HNE shows a time‐dependent decrease of HNE, disappearance from the medium within 2 h and concomitant formation of two unconjugated (phase I) metabolites, 4‐hydroxy‐2‐nonenoic acid (HNA) and 1,4‐dihydroxy‐2‐nonene (DHN), which were both identified and quantified by LC and accounted for 48.8 ± 4.6% of the HNE dose. Four additional metabolites were identified in the

Who reads Detoxification of 4‐hydroxynonenal (HNE) in keratinocytes: characterization of conjugated metabolites by liquid chromatography/electrospray ionization tandem mass spectrometry?

Author

Giancarlo Aldini; Paola Granata; Marica Orioli; Enzo Santaniello; Marina Carini

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)

Published

2003

Language

EN

Field

Chemistry (Physical Sciences)