Opening book details…
Can I read Chaperone function of mutant versions of αA- and αB-crystallin prepared to pinpoint chaperone binding sites on EtoBox?
Chaperone function of mutant versions of αA- and αB-crystallin prepared to pinpoint chaperone binding sites by Barry K. Derham; Martinus A. M. van Boekel; Paul J. Muchowski; John I. Clark; Joseph Horwitz; Henry W. Hepburne-Scott; W. W. de Jong; M. James C. Crabbe; John J. Harding is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Chaperone function of mutant versions of αA- and αB-crystallin prepared to pinpoint chaperone binding sites about?
A major stress protein, α‐crystallin, functions as a chaperone. Site‐directed mutagenesis has been used to identify regions of the protein necessary for chaperone function. In this work we have taken some of the previously described mutants produced and assessed their chaperone function by both a traditional heat‐induced aggregation method at elevated temperature and using enzyme methods at 37 °C. In general the different assays gave parallel results indicating that the same property is being measured. Discrepancies were explicable by the heat lability of some mutants. Most mutants had full chaperone function showing the robust nature of α‐crystallin. A mutant corresponding to a minor component of rodent αA‐crystallin, αAins‐crystallin, had decreased chaperone function. Decreased chaperone function was also found for human Ser139→ Arg, Thr144→Arg, Ser59→Ala mutants of αB‐crystallin and double mutants Ser45→Ala/Ser59→Ala, Lys103→ Leu/His104→Ile, and Glu110→His/His111→Glu. A mutant Phe27→Arg that was the subject of previous controversy was shown to be fully active at physiological temperatures.
Who reads Chaperone function of mutant versions of αA- and αB-crystallin prepared to pinpoint chaperone binding sites?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Barry K. Derham; Martinus A. M. van Boekel; Paul J. Muchowski; John I. Clark; Joseph Horwitz; Henry W. Hepburne-Scott; W. W. de Jong; M. James C. Crabbe; John J. Harding
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)