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Can I read miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken on EtoBox?
miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken by Zhang, Donghao (author);Ran, Jinshan (author);Li, Jingjing (author);Yu, Chunlin (author);Cui, Zhifu (author);Amevor, Felix Kwame (author);Wang, Yan (author);Jiang, Xiaosong (author);Qiu, Mohan (author);Du, Huarui (author);Zhu, Qing (author);Yang, Chaowu (author);Liu, Yiping (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken about?
The proliferation and differentiation of skeletal muscle satellite cells (SMSCs) play an important role in the development of skeletal muscle. Our previous sequencing data showed that miR-21-5p is one of the most abundant miRNAs in chicken skeletal muscle. Therefore, in this study, the spatiotemporal expression of miR-21-5p and its effects on skeletal muscle development of chickens were explored using in vitro cultured SMSCs as a model. The results in this study showed that miR-21-5p was highly expressed in the skeletal muscle of chickens. The overexpression of miR-21-5p promoted the proliferation of SMSCs as evidenced by increased cell viability, increased cell number in the proliferative phase, and increased mRNA and protein expression of proliferation markers including PCNA, CDK2, and CCND1. Moreover, it was revealed that miR-21-5p promotes the formation of myotubes by modulating the expression of myogenic markers including MyoG, MyoD, and MyHC, whereas knockdown of miR-21-5p showed the opposite result. Gene prediction and dual fluorescence analysis confirmed that KLF3 was one of the direct target genes of miR-21-5p. We confirmed that, contrary to the function of miR-21-5p, KLF3
Who reads miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Zhang, Donghao (author);Ran, Jinshan (author);Li, Jingjing (author);Yu, Chunlin (author);Cui, Zhifu (author);Amevor, Felix Kwame (author);Wang, Yan (author);Jiang, Xiaosong (author);Qiu, Mohan (author);Du, Huarui (author);Zhu, Qing (author);Yang, Chaowu (author);Liu, Yiping (author)
- Publisher
- MDPI AG
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)