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Metabolic Flux Analysis of Hepatocyte Function in Hormone- and Amino Acid-supplemented Plasma by Christina Chan; François Berthiaume; Kyongbum Lee; Martin L. Yarmush is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Metabolic Flux Analysis of Hepatocyte Function in Hormone- and Amino Acid-supplemented Plasma about?

Understanding the metabolic and regulatory pathways of hepatocytes is important for biotechnological applications involving liver cells. Previous attempts to culture hepatocytes in plasma yielded poor functional results. Recently we reported that hormone (insulin and hydrocortisone) and amino acid supplementation reduces intracellular lipid accumulation and restores liver-specific function in hepatocytes exposed to heparinized human plasma. In the current study, we performed metabolic flux analysis (MFA) using a simplified metabolic network model of cultured hepatocytes to quantitively estimate the changes in lipid metabolism and relevant intracellular pathways in response to hormone and amino acid supplementation. The model accounts for the majority of central carbon and nitrogen metabolism, and assumes pseudo-steady-state with no metabolic futile cycles. We found that beta-oxidation and tricarboxylic acid (TCA) cycle fluxes were upregulated by both hormone and amino acid supplementation, thus enhancing the rate of lipid oxidation. Concomitantly, hormone and amino acid supplementation increased gluconeogenic fluxes. This, together with an increased rate of glucose clearance, cause

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Author

Christina Chan; François Berthiaume; Kyongbum Lee; Martin L. Yarmush

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 1096-7176)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)