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?-1,3-Glucanase is highly-expressed in laticifers of Hevea brasiliensis by Mee-Len Chye; Ka-Yun Cheung is a Agricultural and Biological Sciences article available to read on EtoBox.

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Clones encoding fl-1,3-glucanase have been isolated from a Hevea c D N A library prepared from the latex ofHevea brasiliensis using a probe Nicotianaplumbaginifolia cDNA encoding fl-l,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic fl-l,3-glucanase showed 68 ~o nucleotide homology to gnl cDNA. Northern blot analysis using the Hevea cDNA as probe detected a mRNA of 1.3 kb which was expressed at higher levels in latex than in leaf. In situ hybridization analysis using petiole sections from Hevea localized the fl-l,3-glucanase mRNA to the laticifer cells. Genomic Southern analysis suggested the presence of a low-copy gene family encoding fl-1,3-glucanases in H. brasiliensis. Multiple isoforms of fl-l,3-glucanases have been isolated from plants [14]. These various forms can be divided into four different classes based on mRNA expression profile, protein isoelectric point and sequence homology. Class I fl-l,3glucanases are pathogen-inducible, basic, vacuolar proteins that are expressed in roots and older leaves [1,2,9,12,15,21]. Class II, Class III and Class IV fl-l,3-glucanases are acidic proteins. Class II and Class III isoforms are indu

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Author

Mee-Len Chye; Ka-Yun Cheung

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1995

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)