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A Cytokine Immunosensor for Multiple Sclerosis Detection Based Upon Label-free Electrochemical Impedance Spectroscopy by Jeffrey T. La Belle; Kinjal Bhavsar; Aaron Fairchild; Anusuya Das; James Sweeney; T.L. Alford; Joseph Wang; Veer P. Bhavanandan; Lokesh Joshi is a Engineering article available to read on EtoBox.

What is A Cytokine Immunosensor for Multiple Sclerosis Detection Based Upon Label-free Electrochemical Impedance Spectroscopy about?

A biosensor for the serum cytokine, interleukin-12 (IL-12), based upon a label-free electrochemical impedance spectroscopy monitoring is described. Overexpression of IL-12 has been correlated to the diagnosis of multiple sclerosis (MS). The prototype biosensor was fabricated on a disposable gold-coated silver ribbon electrode by immobilizing anti-IL-12 monoclonal antibodies (mAbs) onto the surface of the electrode. This technique was advantageous as the silver electrodes provided a more rigid and conductive substrate than thin gold foil electrodes and helped in obtaining more reproducible data when used with the electrode holder. Results indicate that IL-12 can be detected at physiological levels, <100 fM with p<0.05 in a label-free and real-time manner. The cost-effective approach described here can be used for diagnosis of diseases (like MS) with known biomarkers in body fluids and for monitoring physiological levels of biomolecules with healthcare, food, and environmental relevance.

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Author

Jeffrey T. La Belle; Kinjal Bhavsar; Aaron Fairchild; Anusuya Das; James Sweeney; T.L. Alford; Joseph Wang; Veer P. Bhavanandan; Lokesh Joshi

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)