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Uptake of Serotonin by Adult Rat Corpus Callosum Is Partially Reduced by Common Antidepressants by Daniel Reyes-Haro; Guadalupe García-Alcocer; Ricardo Miledi; Jesús García-Colunga is a Neuroscience article available to read on EtoBox.

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## Abstract The corpus callosum (CC) is the main white matter tract involved in interhemispheric brain communication. We establish that uptake of [^3^H]5‐hydroxytryptamine (5‐HT) in CC is partially inhibited by some antidepressants. Slices of the adult rat CC had a high‐affinity uptake of 5‐HT. About 80% of this uptake was Na^+^ dependent, with a Michaelis‐Menten constant, K~m~, of 420 ± 80 nM and a rate of 5‐HT uptake, V~max~, of 9.5 ± 0.8 pmol/mg protein/min. The 5‐HT uptake was reduced ∼60% at pH 5 compared with that at pH 7. Fluoxetine (Prozac) inhibited only 43% of 5‐HT uptake in a concentration‐dependent manner, with an affinity constant, K~i~, of 44.7 ± 10.0 nM. We also studied the effects of other monoamine uptake inhibitors, all at 10 μM, and found that zimelidine, imipramine, and clomipramine inhibited 5‐HT uptake in the CC by ∼30–40%. The fluoxetine‐insensitive 5‐HT uptake was not altered by high concentrations of dopamine plus norepinephrine. The present data show that Na^+^‐dependent 5‐HT uptake occurs in the CC and optic nerve and that this uptake is partially sensitive to antidepressants and probably mediated by the serotonin transporter, which may be relevant during

Who reads Uptake of Serotonin by Adult Rat Corpus Callosum Is Partially Reduced by Common Antidepressants?

Author

Daniel Reyes-Haro; Guadalupe García-Alcocer; Ricardo Miledi; Jesús García-Colunga

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2003

Language

EN

Field

Neuroscience (Life Sciences)