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A new mechanism of gastric epithelial injury induced by acid exposure: The role of Egr-1 and ERK signaling pathways by Mohamed M.M. Abdel-Latif; Henry J. Windle; Anthony Davies; Yuri Volkov; Dermot Kelleher is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A new mechanism of gastric epithelial injury induced by acid exposure: The role of Egr-1 and ERK signaling pathways about?
## Abstract The molecular mechanisms by which gastric acid causes epithelial injury in the stomach and initiates an inflammatory reaction are poorly understood. We aimed in the present study to investigate the role of the early growth response gene Egr‐1 and ERK in gastric epithelial cells following acid exposure, and the signaling pathways involved. Western blotting was used to assess Egr‐1 protein levels in AGS cells. A quantitative measurement of acid‐induced Egr‐1 and ERK translocation was performed using a high content analysis approach. Egr‐1 functionality was assessed by transient transfection with Egr‐1 antisense oligonucleotide. Exposure of AGS cells to acidic conditions induced Egr‐1 protein expression in a pH‐ and time‐dependent manner. Egr‐1 expression was markedly increased as the pH was reduced from pH 7.4 to 6.4. High content analysis of Egr‐1 activation showed acid‐induced Egr‐1 nuclear translocation; a maximum observed at 1–2 h followed by a decline to basal levels beyond 4 h. Acidic pH also activated ERK1/2 phosphorylation, whereas ERK1/2 inhibitors PD98059 and U0216 blocked both acid‐induced Egr‐1 and ERK translocation and expression. Moreover, acid exposure up‐r
Who reads A new mechanism of gastric epithelial injury induced by acid exposure: The role of Egr-1 and ERK signaling pathways?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mohamed M.M. Abdel-Latif; Henry J. Windle; Anthony Davies; Yuri Volkov; Dermot Kelleher
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)