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Localization of epitopes for monoclonal antibodies at the N-terminus of the porcine zona pellucida glycoprotein pZPC by Dr. S. K. Gupta; Edward C. Yurewicz; Abhijit Afzalpurkar; K. V. S. Rao; Douglas A. Gage; H. Wu; A. G. Sacco is a Medicine article available to read on EtoBox.

What is Localization of epitopes for monoclonal antibodies at the N-terminus of the porcine zona pellucida glycoprotein pZPC about?

## Abstract Zona pellucida glycoproteins play an important role in fertilization. In this study, attempts have been made to identify and define epitopes of monoclonal antibodies (mAbs) possessing contraceptive efficacy in vitro. The porcine zona glycoprotein pZPC, a homologue of mouse/human ZP3, was reduced and alkylated and subsequently digested with trypsin. Reverse‐phase HPLC of the tryptic digest yielded twenty two peaks (T1–T22). When tested against mAbs reactive against sequential determinants on pZPC, T11 was immunoreactive with two mAbs, mAb‐455 and mAb‐467, as shown by antigen inhibition ELISA. IC~50~ values of 3.1 nM and 8.6 nM were recorded versus mAb‐455 and mAb‐467 respectively, and approximated the IC~50~ values obtained with intact pZPC. Amino acid analysis, Edman degradation, and FAB‐MS identified T11 as the N‐blocked decapeptide pyro‐Gln‐Pro‐Val‐Trp‐Gln‐Asp‐Glu‐Gly‐Gln‐Arg derived from the N‐terminus of pZPC. Synthesis of overlapping octapeptides further identified VWQDE and WQDE as the minimum motifs with antigenie activity for mAb‐455 and mAb‐467, respectively. Glycine replacement peptides confirmed residues W,Q,E as critical for binding mAb‐455 and W,Q,D,E as cr

Who reads Localization of epitopes for monoclonal antibodies at the N-terminus of the porcine zona pellucida glycoprotein pZPC?

Author

Dr. S. K. Gupta; Edward C. Yurewicz; Abhijit Afzalpurkar; K. V. S. Rao; Douglas A. Gage; H. Wu; A. G. Sacco

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1040-452X)

Published

1995

Language

EN

Field

Medicine (Life Sciences)