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Can I read A collagen-binding SIRPαFc fusion protein for targeted cancer immunotherapy on EtoBox?
A collagen-binding SIRPαFc fusion protein for targeted cancer immunotherapy by Jiayang Liu; Tongyang Xu; Danjie Pan; Jiajun Fan; Yuan Fu; Xiting Huang; Weili Zhao; Xiaochun Dong; Shaohui Zhang; Kudelaidi Kuerban; Xuan Huang; Songna Wang; Huaning Chen; Yunpeng He; Yi Zhun Zhu; Congjun Wang; Li Ye is a Immunology and Microbiology article available to read on EtoBox.
What is A collagen-binding SIRPαFc fusion protein for targeted cancer immunotherapy about?
Collagen is abundant but exposed in tumor due to the abnormal tumor blood vessels, thus is considered as a tumor-specific target. The A3 domain of von Willebrand factor (vWF A3) is a kind of collagen-binding domain (CBD) which could bind collagen specifically. Previously we reported a chemosynthetic CBD-SIRPαFc conjugate, which could block CD47 and derived tumor-targeting ability by CBD. CBD-SIRPαFc conjugate represented improved anti-tumor efficacy with increased MHC II + M1 macrophages, but the uncertain coupling ratio remained a problem. Herein, we produced a vWF A3-SIRPαFc fusion protein through eukaryotic expression system. It was examined at both molecular and cellular levels with its collagen affinity, uninfluenced original affinity to targets and phagocytosis-promoting function compared to unmodified SIRPαFc. Living imaging showed that vWF A3-SIRPαFc fusion protein derived the improved accumulation and retention in tumor than SIRPαFc. In the MC38 allograft model, vWF A3-SIRPαFc demonstrated a superior tumor-suppressing effect, characterized by increased MHC II + M1 macrophages and T cells (particularly CD4 + T cells). These results revealed that vWF A3-SIRPαFc fusion protei
Who reads A collagen-binding SIRPαFc fusion protein for targeted cancer immunotherapy?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Jiayang Liu; Tongyang Xu; Danjie Pan; Jiajun Fan; Yuan Fu; Xiting Huang; Weili Zhao; Xiaochun Dong; Shaohui Zhang; Kudelaidi Kuerban; Xuan Huang; Songna Wang; Huaning Chen; Yunpeng He; Yi Zhun Zhu; Congjun Wang; Li Ye
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)