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T-Cell Differentiation: Methods and Protocols (Methods in Molecular Biology (1514)) by Enrico Lugli is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is T-Cell Differentiation: Methods and Protocols (Methods in Molecular Biology (1514)) about?

This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails, approaches to measure telomerase activity in terminally differentiated T cells, and approaches to define Treg cells at the phenotypic and functional level. The final part of the book is dedicated to the analysis of the differentiation and effector functions of innate T cells, namely the well-known γ/δ T cells, and the recently identified CD8+ mucosal associated invariant T (MAIT) cells. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Who reads T-Cell Differentiation: Methods and Protocols (Methods in Molecular Biology (1514))?

Author

Enrico Lugli

Publisher

Springer New York : Imprint: Humana Press

Published

2017

Language

EN

ISBN

9781493965489

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)