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Spatial and temporal control of transgene expression in vivo using a heat‐sensitive promoter and MRI‐guided focused ultrasound by E. Guilhon; P. Voisin; J. A. de Zwart; B. Quesson; R. Salomir; C. Maurange; V. Bouchaud; P. Smirnov; H. de Verneuil; A. Vekris; P. Canioni; C. T. W. Moonen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Spatial and temporal control of transgene expression in vivo using a heat‐sensitive promoter and MRI‐guided focused ultrasound about?
## Background: Among the techniques used to induce and control gene expression, a non-invasive, physical approach based on local heat in combination with a heat-sensitive promoter represents a promising alternative but requires accurate temperature control in vivo. mri-guided focused ultrasound (mri-fus) with real-time feedback control allows automatic execution of a predefined temperature-time trajectory. the purpose of this study was to demonstrate temporal and spatial control of transgene expression based on a well-defined local hyperthermia generated by mri-fus. ## Methods: Expression of the green fluorescent protein (gfp) marker gene was used. two cell lines were derived from c6 glioma cells. the gfp expression of the first one is under the control of the cmv promoter, whereas it is under the control of the hsp70 promoter in the second one and thus inducible by heat. subcutaneous tumours were generated by injection in immuno-deficient mice and rats. tumours were subjected to temperatures varying from 42 to 50 degrees c for 3 to 25 min controlled by mri-fus and analyzed 24 h after the heat-shock. endogenous hsp70 expression and c6 cell distribution were also analyzed. ## Result
Who reads Spatial and temporal control of transgene expression in vivo using a heat‐sensitive promoter and MRI‐guided focused ultrasound?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- E. Guilhon; P. Voisin; J. A. de Zwart; B. Quesson; R. Salomir; C. Maurange; V. Bouchaud; P. Smirnov; H. de Verneuil; A. Vekris; P. Canioni; C. T. W. Moonen
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)