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γ-tubulin in Chlamydomonas: Characterization of the gene and localization of the gene product in cells by C.D. Silflow; B. Liu; M. LaVoie; E.A. Richardson; B.A. Palevitz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is γ-tubulin in Chlamydomonas: Characterization of the gene and localization of the gene product in cells about?

In addition to their role in nucleating the assembly of axonemal microtubules, basal bodies often are associated with a microtubule organizing center (MTOC) for cytoplasmic microtubules. In an effort to define molecular components of the basal body apparatus in Chlamydomonas reinhardtii, genomic and cDNA clones encoding ␥-tubulin were isolated and sequenced. The gene, present in a single copy in the Chlamydomonas genome, encodes a protein with a predicted molecular mass of 52,161 D and 73% and 65% conservation with ␥-tubulin from higher plants and humans, respectively. To examine the distribution of ␥-tubulin in cells, a polyclonal antibody was raised against two peptides contained within the protein. Immunoblots of Chlamydomonas proteins show a major cross-reaction with a protein of Mr 53,000. In Chlamydomonas cells, the antibody stains the basal body apparatus as two or four spots at the base of the flagella and proximal to the microtubule rootlets. During cell division, two groups of fluorescent dots separate and localize to opposite ends of the mitotic apparatus. They then migrate during cleavage to positions known to be occupied by basal bodies. Changes in ␥-tubulin localizati

Who reads γ-tubulin in Chlamydomonas: Characterization of the gene and localization of the gene product in cells?

Author

C.D. Silflow; B. Liu; M. LaVoie; E.A. Richardson; B.A. Palevitz

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0886-1544)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)