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In vitro transcription of plant RNA polymerase I‐dependent rRNA genes is species‐specific by Hao Fan; Kimitaka Yakura; Masako Miyanishi; Mamoru Sugita; Masahiro Sugiura is a Agricultural and Biological Sciences article available to read on EtoBox.

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An __in vitro__ transcription system derived from tobacco (__Nicotiana tabacum__) cultured cell (BY‐2) nuclei supports transcription of the RNA polymerase I‐dependent tobacco rRNA gene. The transcription initiation site determined __in vitro__ was found at residue A which corresponds to that lying within the consensus sequence surrounding plant pre‐rRNA initiation sites. The tobacco rRNA gene is actively transcribed in the tobacco nuclear extract while the broad bean rRNA gene is inactive in the heterologous system, indicating that plant RNA polymerase I‐dependent transcription is species‐specific.

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Author

Hao Fan; Kimitaka Yakura; Masako Miyanishi; Mamoru Sugita; Masahiro Sugiura

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1995

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)