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Versatile, High-resolution Anterograde Labeling of Vagal Efferent Projections with Dextran Amines by Gary C. Walter; Robert J. Phillips; Elizabeth A. Baronowsky; Terry L. Powley is a Neuroscience article available to read on EtoBox.

What is Versatile, High-resolution Anterograde Labeling of Vagal Efferent Projections with Dextran Amines about?

None of the anterograde tracers used to label and investigate vagal preganglionic neurons projecting to the viscera has proved optimal for routine and extensive labeling of autonomic terminal fields. To identify an alternative tracer protocol, the present experiment evaluated whether dextran conjugates, which have produced superior results in the CNS, might yield widespread and effective labeling of long, fine-caliber vagal efferents in the peripheral nervous system. The dextran conjugates that were evaluated proved reliable and versatile for labeling the motor neuron pool in its entirety, for single- and multiple-labeling protocols, for both conventional and confocal fluorescence microscopy, and for permanent labeling protocols for brightfield microscopy of the projections to the gastrointestinal (GI) tract. Using a standard ABC kit followed by visualization with DAB as the chromagen, Golgi-like labeling of the vagal efferent terminal fields in the GI wall was achieved with the biotinylated dextrans. The definition of individual terminal varicosities was so sharp and detailed that it was routinely practical to examine the relationship of putative vagal efferent contacts (by the cr

Who reads Versatile, High-resolution Anterograde Labeling of Vagal Efferent Projections with Dextran Amines?

Author

Gary C. Walter; Robert J. Phillips; Elizabeth A. Baronowsky; Terry L. Powley

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-0270)

Published

2009

Language

EN

Field

Neuroscience (Life Sciences)