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Rac, a Novel Ras-related Family of Proteins That Are Botulinum Toxin Substrates by J Didsbury; R F Weber; G M Bokoch; T Evans; R Snyderman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Rac, a Novel Ras-related Family of Proteins That Are Botulinum Toxin Substrates about?

A new family of ras-related proteins, designated rac (cas-related C3 botulinum toxin substrate) has been identified. racl and rac2 cDNA clones were isolated from a differentiated HL-60 library and encode proteins that are 92% homologous and share 58% and 26-30% amino acid homology with human rhos and ras, respectively. Nucleotide sequence analysis predicts both racl and rac2 proteins to contain 192 amino acids with molecular masses of 21,450 and 21,429 daltons, respectively. racl and rac2 possess four of the five conserved functional domains in ras associated with binding and hydrolysis of guanine nucleotides. They also contain the COOH-terminal consensus sequence Cys-X-X-X-COOH which localizes ras to the inner plasma membrane and the residues Gly" and Alas8, at which sites mutations elicit transforming potential to ras. The rac transcripts, particularly rac2, display relative myeloid tissue selectivity. Both racl transcripts (2.4 and 1.1 kilobases (kb)) increase when HL-60 cells differentiate to neutrophil-like morphology. In contrast, differentiation of U937 cells to monocytelike morphology causes no change in the 2.4-kb mRNA and a decrease in the 1.1-kb mRNA species. rac2 mRNA (

Who reads Rac, a Novel Ras-related Family of Proteins That Are Botulinum Toxin Substrates?

Author

J Didsbury; R F Weber; G M Bokoch; T Evans; R Snyderman

Publisher

Elsevier BV

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)