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Dendritic and Axonal Targeting of the Vesicular Acetylcholine Transporter to Membranous Cytoplasmic Organelles in Laterodorsal and Pedunculopontine Tegmental Nuclei by Garzn, Miguel; Pickel, Virginia M. is a Neuroscience article available to read on EtoBox.

What is Dendritic and Axonal Targeting of the Vesicular Acetylcholine Transporter to Membranous Cytoplasmic Organelles in Laterodorsal and Pedunculopontine Tegmental Nuclei about?

Autoregulation of cholinergic neurons in the laterodorsal tegmental (LDT) and pedunculopontine (PPT) nuclei has been implicated in many functions, most importantly in drug reinforcement and in the pathophysiology of schizophrenia. This autoregulation is attributed to the release of acetylcholine, but neither the storage or release sites are known. To determine these sites, we used electron microscopy for the immunocytochemical localization of antipeptide antiserum raised against the vesicular acetylcholine transporter (VAchT) that is responsible for the uptake of acetylcholine into storage vesicles. The cellular and subcellular distribution of VAchT was remarkably similar in the two regions by by using each of two methods, immunogold and immunoperoxidase. In both PPT and LDT nuclei, VAchT labeling was seen mainly on membranous organelles including the trans-Golgi network in many somata. VAchT-immunoreactive tubulovesicles resembling saccules of smooth endoplasmic reticulum were often seen near the plasma membrane in dendrites. The VAchT-containing dendrites comprised almost 50% of the labeled profiles (1027/2129) in PPT and LDT nuclei. The remaining VAchT-immunoreactive profiles we

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Author

Garzn, Miguel; Pickel, Virginia M.

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

2000

Language

EN

Field

Neuroscience (Life Sciences)