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Membrane Remodulation and Hyperactivation Are Impaired in Frozen-thawed Sperm of Low-fertility Bulls by Miriama Štiavnická; Petr Hošek; Laura Abril-Parreño; David A. Kenny; Patrick Lonergan; Sean Fair is a Medicine article available to read on EtoBox.

What is Membrane Remodulation and Hyperactivation Are Impaired in Frozen-thawed Sperm of Low-fertility Bulls about?

Bulls used in artificial insemination programmes worldwide undergo quality control checks, which are typically based on the evaluation of sperm motility and morphology. Despite this, some bulls can have lower than expected field fertility and the reasons for this remain to be elucidated. Here we hypothesised that sperm from bulls of varying fertility will differ in their ability to undergo capacitation-related events including an increase in membrane fluidity, protein tyrosine phosphorylation, hyperactivation and the acrosome reaction. Firstly, we used frozen-thawed semen from 10 high-fertility (HF) and 10 low-fertility (LF) bulls, and subjected them to in vitro capacitating conditions, following which sperm viability, membrane fluidity, acrosome integrity and protein tyrosine phosphorylation were assessed using flow cytometry. We then assessed the ability of sperm to undergo hyperactivation (induced using caffeine) utilising computer-assisted sperm analysis, and the acrosome reaction (induced using calcium ionophore) using flow cytometry. When sperm were incubated in capacitating conditions, a higher percentage of viable sperm from HF bulls exhibited high membrane fluidity when co

Who reads Membrane Remodulation and Hyperactivation Are Impaired in Frozen-thawed Sperm of Low-fertility Bulls?

Author

Miriama Štiavnická; Petr Hošek; Laura Abril-Parreño; David A. Kenny; Patrick Lonergan; Sean Fair

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)