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This document describes a tRNA-Dihydrouridine assay to measure the molecular determinants of dihydrouridine synthase activity. The assay involves incubating tRNA samples with potassium hydroxide, then acidifying and adding a solution of N-phenyl-p-phenylenediamine and 2,3-butadione monoxime. The samples are then incubated at high temperatures and iron chloride is added before measuring absorbances at specific wavelengths to detect dihydrouridine levels.

Author

oyamina

Language

EN