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Can I read Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways on EtoBox?
Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways by Dennis Eickelbeck; Till Rudack; Stefan Alexander Tennigkeit; Tatjana Surdin; Raziye Karapinar; Jan‐Claudius Schwitalla; Brix Mücher; Maiia Shulman; Marvin Scherlo; Philipp Althoff; Melanie D. Mark; Klaus Gerwert; Stefan Herlitze is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways about?
## Abstract Optogenetics uses light‐sensitive proteins, so‐called optogenetic tools, for highly precise spatiotemporal control of cellular states and signals. The major limitations of such tools include the overlap of excitation spectra, phototoxicity, and lack of sensitivity. The protein characterized in this study, the Japanese lamprey parapinopsin, which we named UVLamP, is a promising optogenetic tool to overcome these limitations. Using a hybrid strategy combining molecular, cellular, electrophysiological, and computational methods we elucidated a structural model of the dark state and probed the optogenetic potential of UVLamP. Interestingly, it is the first described bistable vertebrate opsin that has a charged amino acid interacting with the Schiff base in the dark state, that has no relevance for its photoreaction. UVLamP is a bistable UV‐sensitive opsin that allows for precise and sustained optogenetic control of G protein‐coupled receptor (GPCR) pathways and can be switched on, but more importantly also off within milliseconds via lowintensity short light pulses. UVLamP exhibits an extremely narrow excitation spectrum in the UV range allowing for sustained activation of
Who reads Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Dennis Eickelbeck; Till Rudack; Stefan Alexander Tennigkeit; Tatjana Surdin; Raziye Karapinar; Jan‐Claudius Schwitalla; Brix Mücher; Maiia Shulman; Marvin Scherlo; Philipp Althoff; Melanie D. Mark; Klaus Gerwert; Stefan Herlitze
- Published
- 2019
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)