Can I read In situ hybridization assay for circular RNA visualization based on padlock probe and rolling circle amplification on EtoBox?
In situ hybridization assay for circular RNA visualization based on padlock probe and rolling circle amplification by Chen Lin; Zhehao Xiao; Xinya Zhang; Guangxiong Wu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is In situ hybridization assay for circular RNA visualization based on padlock probe and rolling circle amplification about?
Circular RNAs (circRNAs) are a class of noncoding RNAs generated by a specific type of RNA alternative splicing called backsplicing through various mechanisms. Recently, thousands of circRNAs have been identified by high-throughput RNA sequencing technologies and bioinformatics analysis. However, the functions of the majority have not been fully elucidated yet. Different tools, such as in situ hybridization, can help visualize the spatial temporal distribution of circRNA molecules, thus assisting the understanding of their biological and physiological functions. Here, we present a simple and straightforward method based on padlock probe hybridization and rolling circle amplification (RCA) for in situ detection of circRNAs. We compared our method with the commercially available BaseScope assay for the detection of Cdr1as in the mouse brain tissue. The result showed that the two methods have achieved comparable detection efficiency, thus demonstrating our padlock probe assay as an alternative yet simple circRNA in situ detection method for the research community.
Who reads In situ hybridization assay for circular RNA visualization based on padlock probe and rolling circle amplification?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Chen Lin; Zhehao Xiao; Xinya Zhang; Guangxiong Wu
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)