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Tunable Ampere phase plate for low dose imaging of biomolecular complexes by Tavabi, Amir H.; Beleggia, Marco; Migunov, Vadim; Savenko, Alexey; Sandin, Sara; Öktem, Ozan; Dunin-Borkowski, Rafal E.; Pozzi, Giulio is a scholarly article available to read on EtoBox.

What is Tunable Ampere phase plate for low dose imaging of biomolecular complexes about?

A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.

Author

Tavabi, Amir H.; Beleggia, Marco; Migunov, Vadim; Savenko, Alexey; Sandin, Sara; Öktem, Ozan; Dunin-Borkowski, Rafal E.; Pozzi, Giulio

Published

2017

Language

EN