About this Medicine article
Whole body detection and imaging of nitric oxide generation in mice following cardiopulmonary arrest: Detection of intrinsic nitrosoheme complexes by Periannan Kuppusamy; Ravi A. Shankar; Valerie M. Roubaud; Jay L. Zweier is a Medicine article available to read on EtoBox.
## Abstract Ischemic tissues generate nitric oxide (NO) by direct reduction of tissue nitrite under the acidic conditions that occur during ischemia. In view of the important implications of this enzyme‐independent mechanism of NO generation on the pathogenesis and treatment of tissue injury, the NO formation in mice subjected to cardiopulmonary arrest was measured and imaged. Real‐time measurement of NO generation was performed by detection of naturally generated NO‐heme complexes in tissues using L‐band electron paramagnetic resonance (EPR) spectroscopy. To distinguish NO generated from nitrite, animals were labeled with isotopically enriched ^15^N‐nitrite. Mice were infused with nitrite (70 mg/kg, intravenous), cardiopulmonary arrest induced by an overdose of phenobarbital, and transferred to the EPR resonator. Measurements of NO generation were performed on the intact animal at the levels of the head, thorax, and abdomen. At the end of 3 hr, major organs were isolated and analyzed for their NO signal. The NO complexes were found to have maximum levels in lung, heart, and liver. Three‐dimensional spatial mapping of the NO complex in the intact animal subjected to cardiopulmonary
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- Author
- Periannan Kuppusamy; Ravi A. Shankar; Valerie M. Roubaud; Jay L. Zweier
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0740-3194)
- Published
- 2001
- Language
- EN
- Field
- Medicine (Health Sciences)