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Fixation and Immunoperoxidase Staining of Oligopeptides After Isoelectric Focusing in Thin Polyacrylamide Slab Gels by P.J. Van Der Sluis; G.J. Boer; C.W. Pool is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Fixation and Immunoperoxidase Staining of Oligopeptides After Isoelectric Focusing in Thin Polyacrylamide Slab Gels about?

In this paper a new method for immunological detection of small peptides after isoelectric focusing in thin (200 micron) polyacrylamide slab gels is presented. The peptides are immobilized immediately after focusing by pressing a sheet of glutaraldehyde-impregnated filter paper onto the gel. By this procedure, the gel adheres to the paper and this press-blot can be stained using immunoperoxidase staining procedures. Using the two-step peroxidase conjugate and the three-step peroxidase-antiperoxidase method, several oligopeptides could be visualized after focusing. The detection limit of this method appears to be in the nanogram range.

Who reads Fixation and Immunoperoxidase Staining of Oligopeptides After Isoelectric Focusing in Thin Polyacrylamide Slab Gels?

Author

P.J. Van Der Sluis; G.J. Boer; C.W. Pool

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1983

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)