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Can I read On-line Biological Sample Cleanup for Electrospray Mass Spectrometry Using Sol–gel Columns on EtoBox?
On-line Biological Sample Cleanup for Electrospray Mass Spectrometry Using Sol–gel Columns by Nina Johannesson; Evan Pearce; Maria Dulay; Richard N. Zare; Jonas Bergquist; Karin E. Markides is a Chemistry article available to read on EtoBox.
What is On-line Biological Sample Cleanup for Electrospray Mass Spectrometry Using Sol–gel Columns about?
Using a slight overpressure, a urine sample is loaded onto a monolithic photopolymerized sol-gel column that has been derivatized with hydrophobic carbon chains and then the complex urine matrix is washed with aqueous solution. A buffer containing organic solvent is used to elute the adsorbed peptides by an applied voltage and the sample is then introduced into a mass spectrometer by sheath flow electrospray. The importance of desalting this type of sample is demonstrated by an experiment that shows that the signal intensity of a test solution with neurotensin, sprayed directly into the mass spectrometer, decreased from 4.5 × 10 4 cps to no detectible signal when just 10% urine is added to the sample solution. We suggest that this procedure may find general application for desalting biological samples prior to mass spectrometric analysis.
Who reads On-line Biological Sample Cleanup for Electrospray Mass Spectrometry Using Sol–gel Columns?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Nina Johannesson; Evan Pearce; Maria Dulay; Richard N. Zare; Jonas Bergquist; Karin E. Markides
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2006
- Language
- EN
- Field
- Chemistry (Physical Sciences)