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Immobilization of a functionalized poly(ethylene glycol) onto β‐cyclodextrin‐coated surfaces by formation of inclusion complexes: Application to the coupling of proteins by M. Guerrouache; C. Karakasyan; C. Gaillet; M. Canva; M. C. Millot is a Materials Science article available to read on EtoBox.

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## Abstract The aim of this study was the immobilization of COOH‐modified poly(ethylene glycol) (PEG) layers onto β‐cyclodextrin‐coated surfaces by formation of inclusion complexes, in view of biosensors applications. To this end, PEGs with one phenyladamantyl and one carboxylic end group (Ad‐PEG‐COOH) were prepared according to a three‐step procedure. After modification of PEG with 4‐toluenesulfonylchloride, the reaction of the tosyl intermediate with the alcoholate of 4‐(1‐adamantyl)‐phenol was carried out in tetrahydrofuran to avoid the formation of by‐products. Then, it was shown by high performance liquid chromatography that the association between β‐cyclodextrin cavities and Ad‐PEG‐COOH polymers was not hindered by the presence of the COOH group. Last, the Ad‐PEG‐COOH polymer was immobilized onto β‐cyclodextrin‐coated gold surfaces by formation of inclusion complexes. The immobilization was performed in water, at room temperature, with a rapid kinetics. After activation of COOH groups with __N__‐hydroxysuccinimide, β‐lactoglobulin was coupled to the biocompatible PEG layer. Functionalization of the gold surface with β‐cyclodextrin cavities, immobilization of Ad‐PEG‐COOH onto

Who reads Immobilization of a functionalized poly(ethylene glycol) onto β‐cyclodextrin‐coated surfaces by formation of inclusion complexes: Application to the coupling of proteins?

Author

M. Guerrouache; C. Karakasyan; C. Gaillet; M. Canva; M. C. Millot

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-8995)

Published

2006

Language

EN

Field

Materials Science (Physical Sciences)