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This document provides instructions for estimating the amount of DNA in a tissue sample using a colorimetric assay with diphenylamine. The key steps are: 1. Isolating DNA from a weighed tissue sample using cold 5% trichloroacetic acid to extract and precipitate the DNA. 2. Treating the isolated DNA with acid to convert deoxyribose sugars to hydroxylevulinylaldehyde which reacts with diphenylamine to produce a blue color. 3. Measuring the intensity of the blue color using a spectrophotometer, which is d

Author

Mirunalini Gobinath

Language

EN