Can I read Decellularised intervertebral discs: A potential replacement for degenerate human discs on EtoBox?

Decellularised intervertebral discs: A potential replacement for degenerate human discs by Norbertczak, Halina Teresa; Ingham, Eileen; Fermor, Hazel L.; Wilcox, Ruth K. is a Medicine article available to read on EtoBox.

What is Decellularised intervertebral discs: A potential replacement for degenerate human discs about?

Intervertebral disc (IVD) degeneration is a major cause of back pain. Current surgical interventions have limitations. An alternative approach is to replace degenerated IVDs with a natural biological scaffold. The removal of cellular components from human IVDs should render them nonimmunogenic upon implantation. The aim of this initial proof of technical feasibility study was to develop a decellularization protocol on bovine IVDs with endplates (EPs) and assess protocol performance before application of the protocol to human IVDs with attached EP and vertebral bone (VB). A decellularization protocol based on hypotonic low concentration sodium dodecyl sulfate (0.1% w/v) with proteinase inhibitors, freeze/thaw cycles, and nuclease and sonication treatments was applied to IVDs. Histological, biochemical, and biomechanical comparisons were made between cellular and decellularized tissue. Cell removal from bovine IVDs was demonstrated and total DNA levels of the decellularized inner annulus fibrosus (iAF), outer annulus fibrosus (oAF), and EP were 40.7 (±11.4), 25.9 (±3.8), and 29.3 (±3.1) ng.mg dry tissue weight, respectively ( = 6, ±95% confidence level [CL]). These values were signif

Who reads Decellularised intervertebral discs: A potential replacement for degenerate human discs?

Author

Norbertczak, Halina Teresa; Ingham, Eileen; Fermor, Hazel L.; Wilcox, Ruth K.

Publisher

Mary Ann Liebert; Mary Ann Liebert Inc.; Mary Ann Liebert Inc (ISSN 1937-3384)

Published

2020

Language

EN

Field

Medicine (Health Sciences)