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Basic fibroblast growth factor accumulates in the nuclei of various bFGF-producing cell types by Shoshanna Tessler; Gera Neufeld is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The intracellular localization of basic fibroblast growth factor (bFGF) was studied in BHK‐21 cells transfected with an expression vector containing the complementary DNA (cDNA) of the human bFGF gene (pbFGF). The intracellular location of bFGF was determined using indirect immunofluorescence. The antibodies used were polyclonal antibodies directed against either recombinant human bFGF or recombinant __Xenopus__: bFGF. The nuclei of transfected cells that produce bFGF, but not the nuclei ofuntransfeted cells, were labeled strongly by the antibodies. The nuclear staining was totally abolished when anti‐bFGF antibodies preadsorbed with bFGF were used. Several types of endothelial cells known to produce bFGF were also stained in their nuclei by the antibodies. Nuclear extracts prepared from transfected cells were found to contain bFGF as determined using heparin‐sepharose affinity chromatography, followed by Western blot analysis of fractions, which stimulated the proliferation BHK‐21 cells. The mitogenic cactivity associated with the nuclei was not destroyed when isolated cell nuclei were digested by trypsin. It is therefore likely that the nucleus associated bFGF is intr

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Author

Shoshanna Tessler; Gera Neufeld

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)