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Comparative study of the antioxidant activity of the essential oils of five plants against the H2O2 induced stress in Saccharomyces cerevisiae 1 1 Introduction 2 2 Materials and methods 2 2.1 Chemicals and reagents 2 2.2 Essential oils 2 2.3 Yeast strain and growth conditions 2 2.4 Phytochemical analysis 2 2.4.1 Determination of total phenols 2 2.4.2 Gas chromatographic (GC) 2 2.4.3 GC–MS assay 3 2.5 Antioxidant activity 3 2.5.1 DPPH free radical scavenging assay 3 2.5.2 ABTS+ free radical scavenging assay 3 2.5.3 Reducing power 3 2.6 Cytotoxicity of EOs 3 2.7 Oxidative stress induction 3 2.8 Tolerance determination 3 2.9 Biochemical assays 3 2.9.1 Preparation of cell-free extract 3 2.9.2 Detection of superoxide dismutase (SOD) activity 4 2.9.3 Determination of catalase (CAT) activity 4 2.9.4 Determination of glutathione reductase (GR) activity 4 2.9.5 Determination of lipid peroxidation 4 2.9.6 Determination of protein carbonyl content (PCO) 4 2.9.7 Succinate dehydrogenase (SDH) activity assay 4 2.10 Statistical analysis 4 3 Results 4 3.1 Chemical composition of EOs 4 3.2 In vitro antioxidant activity of EOs 4 3.3 Sensitivity of S. Cerevisiae to EOs 4 3.4 Effect of EOs on cytotoxi

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nonfiction