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Condensate Cooperativity Underlies Transgenerational Gene Silencing by Zhenzhen Du; Kun Shi; Jordan S. Brown; Tao He; Wei-Sheng Wu; Ying Zhang; Heng-Chi Lee; Donglei Zhang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Condensate Cooperativity Underlies Transgenerational Gene Silencing about?
## SUMMARY Biomolecular condensates have been shown to interact __in vivo__, yet it is unclear whether these interactions are functionally meaningful. Here, we demonstrate that cooperativity between two distinct condensates—germ granules and P bodies—is required for transgenerational gene silencing in __C. elegans__. We find that P bodies form a coating around perinuclear germ granules and that P body components CGH-1/DDX6 and CAR-1/LSM14 are required for germ granules to organize into sub-compartments and concentrate small RNA silencing factors. Functionally, while the P body mutant __cgh-1__ is competent to initially trigger gene silencing, it is unable to propagate the silencing to subsequent generations. Mechanistically, we trace this loss of transgenerational silencing to defects in amplifying secondary small RNAs and the stability of WAGO-4 Argonaute, both known carriers of gene silencing memories. Together, these data reveal that cooperation between condensates results in an emergent capability of germ cells to establish heritable memory.
Who reads Condensate Cooperativity Underlies Transgenerational Gene Silencing?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Zhenzhen Du; Kun Shi; Jordan S. Brown; Tao He; Wei-Sheng Wu; Ying Zhang; Heng-Chi Lee; Donglei Zhang
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)