Can I read Rare PIK3CA hotspot mutations in carcinomas of the biliary tract on EtoBox?
Rare PIK3CA hotspot mutations in carcinomas of the biliary tract by Marc‐Oliver Riener; Marion Bawohl; Pierre‐Alain Clavien; Wolfram Jochum is a Medicine article available to read on EtoBox.
What is Rare PIK3CA hotspot mutations in carcinomas of the biliary tract about?
## Abstract Somatic mutations of the __PIK3CA__ gene, which encodes the p110α catalytic subunit of phosphatidylinositol 3‐kinase (PI3K), are frequent in various cancer types. The majority of mutations cluster at hotspots within exons 9 and 20, which encode the helical and kinase domains of p110α. __PIK3CA__ mutations in bile duct and gallbladder carcinomas have not been reported yet. In this study, we analysed 118 carcinomas of the biliary tract and the liver (45 intra‐ and extrahepatic cholangiocarcinomas (CCA), 23 gallbladder carcinomas, 50 hepatocellular carcinomas) for __PIK3CA__ hotspot mutations using polymerase chain reaction and direct DNA sequencing. __PIK3CA__ missense mutations were found in one of 11 intrahepatic CCA (E545K, 9%), one of 23 gallbladder carcinomas (E542K, 4%), and one of 50 hepatocellular carcinomas (H1047R, 2%). All three mutations represent hotspot mutations, which also occur in other cancer types. PI3K pathway activation in hepato‐biliary carcinomas was analyzed using immunohistochemistry for the downstream targets eIF4‐E and phosphorylated 4E‐BP1 on tissue microarrays. eIF4‐E expression was found in 3/13 intrahepatic CCA (23%), 9/38 extrahepatic CCA (
Who reads Rare PIK3CA hotspot mutations in carcinomas of the biliary tract?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Marc‐Oliver Riener; Marion Bawohl; Pierre‐Alain Clavien; Wolfram Jochum
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 1045-2257)
- Published
- 2008
- Language
- EN
- Field
- Medicine (Life Sciences)