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Differential Effect of Thioacetamide on Hepatic Methionine Adenosyltransferase Expression in the Rat by Zong-Zhi Huang; José M. Mato; Gary Kanel; Shelly C. Lu is a Medicine article available to read on EtoBox.

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Liver-specific and non-liver-specific methionine adenosyltransferase (MAT) are products of two genes, MAT1A and MAT2A, respectively, that catalyze the formation of S-adenosylmethionine (SAM), the principal methyl donor. Mature liver expresses mainly MAT1A. We showed a switch from MAT1A to MAT2A gene expression in human liver cancer cells that may offer a growth advantage. To gain a better understanding of the chronology and significance of the change in MAT expression, we examined changes in hepatic MAT expression after acute treatment of rats with a hepatocarcinogen, thioacetamide (TAA). TAA treatment for 3 weeks did not change the MAT1A mRNA level but reduced the liver-specific MAT protein level to below 30% of control. TAA also acutely reduced the activity of liverspecific MAT when added to normal liver homogenates. In contrast, both the mRNA and protein levels of non-liverspecific MAT were induced. Because liver-specific MAT exhibits a much higher K m for methionine (mmol/L) than non-liver-specific MAT (Ϸ10 mol/L), MAT activity was decreased at 5 mmol/L but increased at 20 mol/L methionine concentration. The SAM level, SAM-to-S-adenosylhomocysteine (SAH) ratio, and DNA methylat

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Author

Zong-Zhi Huang; José M. Mato; Gary Kanel; Shelly C. Lu

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

1999

Language

EN

Field

Medicine (Health Sciences)