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Second Line Injectable Drug Resistance and Associated Genetic Mutations in Newly Diagnosed Cases of Multidrug-Resistant Tuberculosis by Vangala Ramakrishna; Pravin Kumar Singh; Shantanu Prakash; Amita Jain is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Second Line Injectable Drug Resistance and Associated Genetic Mutations in Newly Diagnosed Cases of Multidrug-Resistant Tuberculosis about?

To investigate the phenotypic and genotypic profile of multidrug-resistant (MDR) (MTB) clinical isolates with reference to second-line injectable drugs (SLIDs). A total of 110 MTB isolates, recovered consecutively from confirmed MDR-TB patients between March and June 2016, were included in this study. Phenotypic drug susceptibility testing against SLIDs (Kanamycin, Amikacin, and Capreomycin) and Ofloxacin (OFX) was performed using the MGIT 960 system. For genotypic analysis, SLID/(s) resistant ( = 13) and susceptible isolates ( = 26) were subjected to PCR and DNA sequencing for , (promoter region), and A loci of MTB. Furthermore, the identified genetic mutations were analyzed with respect to its significance in detecting phenotypic resistance. Among the 110 analyzed isolates, phenotypic resistance to OFX, SLIDs, and to both was 59.1%, 11.8%, and 10.0%, respectively. Out of a total 13 SLID/(s) resistant isolates, 10 had mutations (including two novel mutations) in one or more of the targeted genes. Only one SLID susceptible MTB isolate showed mutation in the targeted region. In SLID resistant isolates, most frequent mutation detected was C-12T under promoter region (46.1%). Mutation

Who reads Second Line Injectable Drug Resistance and Associated Genetic Mutations in Newly Diagnosed Cases of Multidrug-Resistant Tuberculosis?

Author

Vangala Ramakrishna; Pravin Kumar Singh; Shantanu Prakash; Amita Jain

Publisher

Mary Ann Liebert; Mary Ann Liebert Inc.; Mary Ann Liebert Inc (ISSN 1076-6294)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)