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Simultaneous Analysis of Hemoglobin Adducts of Acrylamide and Glycidamide by Gas Chromatography–Mass Spectrometry by Hermes Licea Pérez; Hae-Kwan Cheong; Jeong Sun Yang; Siv Osterman-Golkar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Simultaneous Analysis of Hemoglobin Adducts of Acrylamide and Glycidamide by Gas Chromatography–Mass Spectrometry about?

Acrylamide (AA) is a carcinogen in experimental animals. Glycidamide (GA), formed by metabolic epoxidation of AA, is believed to be responsible for the carcinogenicity of AA. Occupational exposure to AA has been assessed earlier by measurement of its adducts with N-terminal valine in hemoglobin. A background of AA adducts [N-(2-carbamoylethyl)valine (AAVal), about 30 pmol/g globin] was found in individuals without known exposure to the compound. The method previously available for adducts of GA only allowed analysis of samples from highly exposed individuals and showed similar levels of AAVal and adducts of GA [N-(2-hydroxy-2-carbamoylethyl)valine (GAVal)]. We have developed a sensitive method for simultaneous quantification of adducts of GA and AA, which is suitable down to low exposure levels. The method is based on the so-called modified Edman method, where globin is reacted with pentafluorophenyl isothiocyanate under neutral conditions. The valine adducts are then extracted in the form of pentafluorophenylthiohydantoin (PFPTH) derivatives. The analytical procedure included reaction of the PFPTH derivatives with acetic anhydride in order to protect the hydroxyl group of GAVal. T

Who reads Simultaneous Analysis of Hemoglobin Adducts of Acrylamide and Glycidamide by Gas Chromatography–Mass Spectrometry?

Author

Hermes Licea Pérez; Hae-Kwan Cheong; Jeong Sun Yang; Siv Osterman-Golkar

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)