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Can I read Auxin-mediated Protein Depletion for Metabolic Engineering in Terpene-producing Yeast on EtoBox?
Auxin-mediated Protein Depletion for Metabolic Engineering in Terpene-producing Yeast by Lu, Zeyu (author);Peng, Bingyin (author);Ebert, Birgitta E. (author);Dumsday, Geoff (author);Vickers, Claudia E. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Auxin-mediated Protein Depletion for Metabolic Engineering in Terpene-producing Yeast about?
## Abstract In metabolic engineering, loss-of-function experiments are used to understand and optimise metabolism. A conditional gene inactivation tool is required when gene deletion is lethal or detrimental to growth. Here, we exploit auxin-inducible protein degradation as a metabolic engineering approach in yeast. We demonstrate its effectiveness using terpenoid production. First, we target an essential prenyl-pyrophosphate metabolism protein, farnesyl pyrophosphate synthase (Erg20p). Degradation successfully redirects metabolic flux toward monoterpene (C10) production. Second, depleting hexokinase-2, a key protein in glucose signalling transduction, lifts glucose repression and boosts production of sesquiterpene (C15) nerolidol to 3.5 g L^−1^ in flask cultivation. Third, depleting acetyl-CoA carboxylase (Acc1p), another essential protein, delivers growth arrest without diminishing production capacity in nerolidol-producing yeast, providing a strategy to decouple growth and production. These studies demonstrate auxin-mediated protein degradation as an advanced tool for metabolic engineering. It also has potential for broader metabolic perturbation studies to better understand met
Who reads Auxin-mediated Protein Depletion for Metabolic Engineering in Terpene-producing Yeast?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Lu, Zeyu (author);Peng, Bingyin (author);Ebert, Birgitta E. (author);Dumsday, Geoff (author);Vickers, Claudia E. (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)