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Nanoparticle Administration Method in Cell Culture Alters Particle-cell Interaction by Thomas L. Moore; Dominic A. Urban; Laura Rodriguez-Lorenzo; Ana Milosevic; Federica Crippa; Miguel Spuch-Calvar; Sandor Balog; Barbara Rothen-Rutishauser; Marco Lattuada; Alke Petri-Fink is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Nanoparticle Administration Method in Cell Culture Alters Particle-cell Interaction about?
## Abstract As a highly interdisciplinary field, working with nanoparticles in a biomedical context requires a robust understanding of soft matter physics, colloidal behaviors, nano-characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out to understand how__in vitro__technique, specifically the way we administer particles in 2D culture, can influence experimental outcomes. Gold nanoparticles coated with poly(vinylpyrrolidone) were added to J774A.1 mouse monocyte/macrophage cultures as either a concentrated bolus, a bolus then mixed via aspiration, or pre-mixed in cell culture media. Particle-cell interaction was monitored via inductively coupled plasma-optical emission spectroscopy and we found that particles administered in a concentrated dose interacted more with cells compared to the pre-mixed administration method. Spectroscopy studies reveal that the initial formation of the protein corona upon introduction to cell culture media may be responsible for the differences in particle-cell interaction. Modeling of particle deposition using the__in vitro
Who reads Nanoparticle Administration Method in Cell Culture Alters Particle-cell Interaction?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Thomas L. Moore; Dominic A. Urban; Laura Rodriguez-Lorenzo; Ana Milosevic; Federica Crippa; Miguel Spuch-Calvar; Sandor Balog; Barbara Rothen-Rutishauser; Marco Lattuada; Alke Petri-Fink
- Publisher
- Springer Science and Business Media LLC
- Published
- 2019
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)