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RhoA/Rho kinase‐mediated Ca^2+^ sensitization in the contraction of human prostate by Ryosuke Takahashi; Junji Nishimura; Narihito Seki; Takakazu Yunoki; Toshihisa Tomoda; Hideo Kanaide; Seiji Naito is a Medicine article available to read on EtoBox.

What is RhoA/Rho kinase‐mediated Ca^2+^ sensitization in the contraction of human prostate about?

## Abstract ## Aims The contractile mechanisms of prostatic smooth muscle have been extensively investigated at the receptor level. However, the intracellular mechanisms have not yet been fully elucidated, especially in human tissue. In the present study, we examined the functional role of RhoA/Rho kinase (ROCK), one of the major intracellular molecules involved in smooth muscle contraction, in the contraction of the human prostate. ## Methods Ring preparations made of cultured human prostatic stromal cells (CHPSCs) or fresh human prostatic tissue was used for an isometric tension study. Gene transfer using baculovirus vector and α‐toxin permeabilized preparations were also used. ## Results RhoA, ROCK I and ROCK II proteins were all expressed in CHPSCs and fresh human prostatic tissue. In CHPSCs ring preparations, the contraction induced by endothelin (ET)‐1 was enhanced by over‐expression of RhoA and inhibited by ROCK inhibitor. In α‐toxin permeabilized preparations, ET‐1 or GTP‐γS induced an additional contraction at a constant [Ca^2+^]~i~, that was inhibited by ROCK inhibitor. In fresh human prostatic tissue, norepinephrine (NE)‐induced contraction was inhibited by ROCK inhibito

Who reads RhoA/Rho kinase‐mediated Ca^2+^ sensitization in the contraction of human prostate?

Author

Ryosuke Takahashi; Junji Nishimura; Narihito Seki; Takakazu Yunoki; Toshihisa Tomoda; Hideo Kanaide; Seiji Naito

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0733-2467)

Published

2007

Language

EN

Field

Medicine (Health Sciences)