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Srebf1 Controls Midbrain Dopaminergic Neurogenesis by Enrique M. Toledo; Shanzheng Yang; Daniel Gyllborg; Kim E. van Wijk; Indranil Sinha; Manuel Varas-Godoy; Christopher L. Grigsby; Peter Lönnerberg; Saiful Islam; Knut R. Steffensen; Sten Linnarsson; Ernest Arenas is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Srebf1 Controls Midbrain Dopaminergic Neurogenesis about?

Liver X receptors (LXRs) and their ligands are potent regulators of midbrain dopaminergic (mDA) neurogenesis and differentiation. However, the molecular mechanisms by which LXRs control these functions remain to be elucidated. Here, we perform a combined transcriptome and chromatin immunoprecipitation sequencing (ChIP-seq) analysis of midbrain cells after LXR activation, followed by bioinformatic analysis to elucidate the transcriptional networks controlling mDA neurogenesis. Our results identify the basic helix-loop-helix transcription factor sterol regulatory element binding protein 1 (SREBP1) as part of a cluster of proneural transcription factors in radial glia and as a regulator of transcription factors controlling mDA neurogenesis, such as Foxa2. Moreover, loss- and gain-of-function experiments in vitro and in vivo demonstrate that Srebf1 is both required and sufficient for mDA neurogenesis. Our data, thus, identify Srebf1 as a central player in mDA neurogenesis.

Who reads Srebf1 Controls Midbrain Dopaminergic Neurogenesis?

Author

Enrique M. Toledo; Shanzheng Yang; Daniel Gyllborg; Kim E. van Wijk; Indranil Sinha; Manuel Varas-Godoy; Christopher L. Grigsby; Peter Lönnerberg; Saiful Islam; Knut R. Steffensen; Sten Linnarsson; Ernest Arenas

Publisher

Elsevier ; Cell Press; Amsterdam: Elsevier; Research Square; Elsevier BV (ISSN 2211-1247)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)